The Cell Cycle Analysis | 合法醫療器材資訊網

ThemostcommonmethodforassessingthecellcycleistouseflowcytometrytomeasurecellularDNAcontent.Duringthisprocess,afluorescentdyethatbinds ...

Introduction

The cell cycle is the process by which eukaryotic cells duplicate and divide. The cell cycle consists of two specific and distinct phases: interphase, consisting of G1 (Gap 1), S (synthesis), and G2 (Gap 2), and the mitotic phase; M (mitosis) (Figure 1). During interphase, the cell grows (G1), accumulates the energy necessary for duplication, replicates cellular DNA (S), and prepares to divide (G2) [2]. At this point, the cell enters the M phase, which is divided into two tightly regulated stages: mitosis and cytokinesis. During mitosis, a parent cells chromosomes are divided between two sister cells. In cytokinesis, the division of the cytoplasm occurs, leading to the formation of two distinct daughter cells. Each phase of the cell cycle is tightly regulated, and checkpoints exist to detect potential DNA damage and allow it to be repaired before a cell divides. If the damage cannot be ...