Comparison of API 20E and invA PCR for Identification of ... | 合法醫療器材資訊網

由DMNucera著作·2006·被引用94次—TheAPI20Ediagnostic,whichdetects20biochemicalreactions,isatraditionalmethodfortheidentificationofSalmonellaentericaandotherEnterobacteriaceae( ...

API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.

The API 20E diagnostic, which detects 20 biochemical reactions, is a traditional method for the identification of Salmonella enterica and other Enterobacteriaceae (11). Previous studies of API 20E have reported both good (14, 16, 20) and inaccurate (1, 2, 9, 18) sample classifications. Genetic identification systems may improve Salmonella identification (8, 22). An alternative is the highly sensitive and specific invA PCR (5, 13). invA PC...