Indirect Antiglobulin Test (IAT | 合法醫療器材資訊網

TheAHGserumthatisroutinelyusedbymostlabsforpretransfusionIATsismonospecificanti-IgG.WhendoingDATstodetectinvivosensitizationwithIgGorC3, ...

The antiglobulin (AHG) test was invented by Coombs, Race and Mourant in 1945 and is sometimes called the Coombs test after its main inventor. It is the single most important test we have for detection of red cell antibodies.

Because antibodies are gamma globulins, an antibody to gamma globulin can form bridges between red cells sensitized with antibody and cause them to agglutinate (Figure 3-1[1]). Since most incomplete antibodies are IgG, polyspecific AHG serum contains anti-IgG. Because some IgG and IgM antibodies also cause C3 to attach to red cells, polyspecific AHG serum also contains anti-C3, which can cause C3-coated red cells to agglutinate. The AHG serum that is routinely used by most labs for pretransfusion IATs is monospecific anti-IgG. When doing DATs to detect in vivo sensitization with IgG or C3, blood banks must use polyspecific AHG serum containing anti-IgG, anti-C3b, and anti-C3d.