Antibody Diluent (ab64211) | 合法醫療器材資訊網

Generalnotes.AntibodyDiluentab64211isusedtodiluteprimaryantibodiestotheirworkingconcentrationsforuseinIHC.Itcanalsobeusedforconvenient ...

Antibody Diluent ab64211 is used to dilute primary antibodies to their working concentrations for use in IHC. It can also be used for convenient storage of diluted primary antibodies.

The antibody diluent is formulated to reduce background and non-specific binding of the primary antibody. In most cases, antibodies can be stored in the diluent, at their working concentrations for IHC, for up to 24 months at 2-8°C.

The diluent is based on a Tris buffer, and contains a stabilizing carrier protein, a wetting agent to reduce surface tension, an anti-microbial agent, and a green dye. It should not be used with HRP-conjugated primary antibodies.

IHC protocol suitable for use with Antibody Diluent ab64211:For fluorescent IHC, skip step 3, incubate with fluorescent dye conjugated secondary at step 6, skip rest of steps, and mount with anti-fade mounting medium.[1]

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