KAPA HiFi HotStart ReadyMix | 合法醫療器材資訊網

RocheKAPAHiFiHotStartReadyMixPCRKitusedinNGSlibraryamplification....KAPAHiFiDNAPolymerase(HotStartandReadyMixformulation)isanovel,...Thispreventsnonspecificamplificationduringreactionsetup,increases ...

KAPA HiFi HotStart DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA without accessing accessory proteins or binding domains.

The intrinsic high processivity of the enzyme results in significant improvement in yield, speed, and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. The enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency.

In the ReadyMix PCR Kit, the enzyme is supplied in a convenient 2X ReadyMix format, containing all reaction components except primers and template. The ReadyMix contains KAPA ...