PDH Quick Protocol | 合法醫療器材資訊網

If using >5 ml of bacterial cells, centrifuge at 16-20,000 x g for 5-8 minutes. During centrifugation, place a PDH Column in a 2 ml Collection. Tube. 5. DNA Binding. Read More

PD1, PD2, PD3 Buffers; TrueBlue Lysis Buffer; W1 Buffer; Wash Buffer; Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C); RNase A; PDH Columns; 2 ml Collection​ ... Read More

Transfer the dried. PDH Column to a new 1.5 ml microcentrifuge tube. NOTE: Perform Wash Buffer steps twice for salt sensitive downstream applications. 7. Elution. Read More

PE1, PE2, PE3, PE4, PE5 Buffers; TrueBlue Lysis Buffer; W1 Buffer; Wash Buffer; Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C); RNase A; PDH Columns; 2 ml ... Read More

2017年11月13日 — 上述文章中提到，核酸萃取的方式有許多種，包括管柱萃取純化法(Column Purification)、試劑萃取純化法(有機溶劑萃取法與非有機溶劑萃取法) ... Read More

For Standard Plasmid DNA Purification Add 600 µl of Wash Buffer (make sure absolute ethanol was added) into the PDH Column. Centrifuge at 14-16,000 x g for. Read More

Transfer the dried. PDH Column to a new 1.5 ml microcentrifuge tube. NOTE: Perform Wash Buffer steps twice for salt sensitive downstream applications. 7. Elution. Read More

Geneaid PDH Columns are specially designed and pretreated to ensure high ... plasmid DNA in the lysate binds to the glass fiber matrix of the spin column. Read More