FACS buffer composition | 合法醫療器材資訊網

2017年5月12日—5IngredientstoConsiderinFACSBuffer·Introduction·1-UseCa/Mg2+freePBS·2-SupplementwithFCS(1-10%)orBSA(0.1-1%)·3-Include ...

Serum proteins protect cells from apoptosis, prevent non-specific staining and also prevent cells from sticking to the FACS tubes. However, a high concentration of serum can contribute to auto-fluorescence, therefore the optimum serum level should be pilot tested beforehand.

EDTA also prevents cation based cell to cell adhesion and so should be included in the buffer if dealing with sticky and adherent cells like macrophages. However, the concentration of EDTA should be titrated first as high levels can be cytotoxic and/or cytocidal.

Dead cells release their contents including DNA into the medium which can lead to cell clumping and related problems. If the sample has a high percentage of dead cells – ideally, the dead cel...