Fluorescence activated cell sorting of live cells | 合法醫療器材資訊網

Fluorescenceactivatedcellsorting(FACS)oflivecellsseparatesapopulationofcellsintosub-populationsbasedonfluorescentlabeling.Sortinginvolvesmore ...

​A description of fluorescence-activated cell sorting of live cell populations.

Print this protocol.[1]

Fluorescence-activated cell sorting (FACS) of live cells separates a population of cells into subpopulations based on fluorescent labeling. Sorting involves more complex mechanisms in the flow cytometer compared to a non-sorting analysis.

Cells stained using fluorophore-conjugated antibodies can be separated from one another depending on which fluorophore they have been stained with. For example, a cell expressing one cell marker may be detected using a FITC-conjugated antibody that recognizes the marker, while another cell type expressing a different marker could be detected using a PE-conjugated antibody specific to that marker. This is the fundamental principle of flow cytometry.

Cells stained using fluorophore-conjugated antibodies can be ...