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Automate Your qPCR Workflow | 合法醫療器材資訊網

Automate Your qPCR Workflow

Weprovidecutting-edgeautomatedsolutionsthatcovereachstepofyourreal-​timePCRworkflow—fromsampletoresult.Regardlessofwhetheryouworkin ...

 Automate Your qPCR Workflow

The following paramenters can be optimized to increase transfection efficiency when using Effectene Transfection Reagent[1]:

Optimize the Effectene Reagent to DNA ratio

If the ratio of Effectene Reagent to DNA is suboptimal, the overall charge of the complexes may be negative, neutral or strongly positive, which can lead to inefficient adsorption to the cell surface. Optimize the Effectene Reagent to DNA ratio according to the section " Transfection Optimization" of the Effectene Transfection Reagent Handbook[2].

Increase the amount of Effectene-DNA complex

If the transfection efficiency is lower than expected and cytotoxicity acceptably low, increase the overall amount of Effectene-DNA complexes. See the pipetting scheme in the section "Transfection Optimization" of the Effectene Transfection Reagent Handbo...

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