IHC antigen retrieval protocol | 合法醫療器材資訊網
![IHC antigen retrieval protocol](https://i.imgur.com/6y29Lx7.jpg)
Buffersolutionsforheat-inducedepitoperetrieval·Tri-sodiumcitrate(dihydrate)2.94g·Distilledwater1L·Mixtodissolve.AdjustpHto6.0with1NHCl.·Add0.5 ...
![IHC antigen retrieval protocol](https://i.imgur.com/6y29Lx7.jpg)
The following solutions are three of the more popular buffers for HIER. In the absence of datasheet information, choice of retrieval buffer is best accomplished by trial.
Sodium citrate buffer (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
Tri-sodium citrate (dihydrate) 2.94 g Distilled water 1 L Mix to dissolve. Adjust pH to 6.0 with 1N HCl. Add 0.5 mL Tween 20 and mix well. Store at room temperature for 3 months or at 4°C for longer storage1 mM EDTA, pH 8.0
EDTA 0.37 g Distilled water 1 L Adjust to pH 8.0 with NaOH Store at room temperature for 3 monthsTris-EDTA buffer (10 mM Tris base, 1 mM EDTA solution, 0.05% Tween 20, pH 9.0)
Tris 1.21 g EDTA 0.37 g Distilled water 1 L Mix to dissolve. Adjust pH ...Citrate Buffer (0.1 M | 合法醫療器材資訊網
Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe | 合法醫療器材資訊網
Citrate Buffer Antigen Retrieval Protocol | 合法醫療器材資訊網
Citrate Buffer Antigen Retrieval Protocol | 合法醫療器材資訊網
Citrate | 合法醫療器材資訊網
IHC antigen retrieval protocol | 合法醫療器材資訊網
IHC Protocols | 合法醫療器材資訊網
Sodium citrate buffer | 合法醫療器材資訊網
Sodium citrate buffer vs. citrate buffer? | 合法醫療器材資訊網
Tris | 合法醫療器材資訊網
![](https://i.imgur.com/6y29Lx7.jpg)
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